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From SARS to MERS, thrusting coronaviruses into the spotlight

In order to recover GST-tagged protein from the complicated mixture of proteins, the fusion protein is incubated with agarose beads coupled to glutathione (GSH-agarose, see picture below), what leads to efficient precipitation of GST-fusion protein

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Narboux-Neme N, Pavone LM, Avallone L, Zhuang X, Gaspar P

Severe reactions like the one documented in published case reports can take up to a month to fully resolve, though these are uncommon

Before we dive into protocols, lets establish a clear baseline